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Amaxa
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Image Search Results
Journal: Oncotarget
Article Title: HDAC2 deregulation in tumorigenesis is causally connected to repression of immune modulation and defense escape
doi:
Figure Lengend Snippet: A. Western blot assays of HDAC1 and 2, and real-time PCR for HDAC2 silencing validation. B. Left: Proliferation curve at 24, 48 and 72 hours for sh2 and scr clones. Right: Western blot of acetylated histones H3, H3K56 and H4K16. ERK1 was used for normalization C. Proliferation curve relative to MDA-MB231 cell line at longer times, showing scr clone (green), sh2 clone (blue) and background (red). D. Migration curve at 24 hours in MDA-MB231 cell line, showing scr clone (blue), sh2 clone (magenta) and background (green).
Article Snippet: In stable transfection experiments U937 cells were used at a concentration of 1 × 10 6 per mL and were stably transfected by nucleofection using the Amaxa®
Techniques: Western Blot, Real-time Polymerase Chain Reaction, Clone Assay, Migration
Journal: Oncotarget
Article Title: HDAC2 deregulation in tumorigenesis is causally connected to repression of immune modulation and defense escape
doi:
Figure Lengend Snippet: A-B. Real-time PCR performed on HLA-DRA and HLA-DPA1 genes in 6 different AML patients. CD34+ cells were used as further control. Data show mean values from three parallel experiments with error bars showing standard deviations above each column. C. HLA-DR-DP expression level measured by FACS analyses in U937 and the sh2 clone. D. CIITA protein expression levels in sh2 and scr clones. Normalization was performed with ERK1. E. HDAC2 protein expression levels in the scr, sh2 and in HDAC2 rescue clones. Normalization was performed with ERK1. HDAC2 rescue reached about 60% of the scr signal. F. Real time PCR for HLA-DRA gene expression in presence of scr, sh2, HDAC2 rescue (WT) and K11R, D100A, H142D HDAC2 mutants. The data show the mean values from three parallel experiments with error bars showing standard deviations above each column.
Article Snippet: In stable transfection experiments U937 cells were used at a concentration of 1 × 10 6 per mL and were stably transfected by nucleofection using the Amaxa®
Techniques: Real-time Polymerase Chain Reaction, Expressing, Clone Assay